DBS Sampling Can be Used to Stabilize Prodrugs in Drug Discovery Rodent Studies without the Addition of Esterase Inhibitors

Abstract

For these two prodrugs, stability on DBS cards was observed in rat EDTA whole blood for at least 21 days at room temperature as determined by loss of prodrug and appearance of parent. The Whatman FTA Elute cards, treated with reagents that lyse cells, did not offer more stability for the investigated compounds than the Whatman 903 Protein Saver untreated cards.