Journal of Cellular Biochemistry · 2000 · 11 citations · 46 references
Life SciencesGeneticsMolecular BiologyDna ReplicationGenome OrganizationNuclear OrganizationNuclear MatrixPlasmid BiologyPlasmid ReplicationReplication InitiationReplication OriginsNuclear Matrix AssociationNuclear Matrix AttachmentNuclear Matrix ElementsNucleotide-binding MotifPlasmid Replication OriginsSequence-specific Dna BindingTopoisomerase Function In ReplicationTopoisomerase Ii Function
Ten short DNA fragments have been selected from a library of the nuclear matrix-attached DNA (nmDNA) from chicken erythrocytes by their ability to hybridize with the fraction of chicken replication origins isolated by nascent DNA strand extrusion. The primary structure of these fragments has been determined. Five of the sequences contained a topoisomerase II recognition site. Most of the studied DNA fragments also have a common eight-nucleotide motif, GCAGACCG/A. A sequence-specific DNA-binding protein with a MW of 55 kDa that interacted with this motif has been identified. Some of the cloned DNA fragments promoted an increased level of transient plasmid replication in transfected chicken cells. The ability of plasmid bearing nmDNA fragments to replicate correlated directly with their ability to target plasmids to the nuclear matrix compartment.
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