Nutrition and Cancer · 2002 · 54 citations · 23 references
Life SciencesBiochemistryCell BiologyApoptosisCancer PharmacologyCell SignalingAntioxidant DefenseApoptosis RegulationApoptotic SignalingBcl-2 Family ProteinsCaspase ActivationReactive Oxygen SpeciesAntiapoptotic Protein SignalingApoptotic ResponseBcl-2 InhibitionCaspase CleavageCaspase SignalingCaspase-mediated ApoptosisReactive Oxygen Species-induced ApoptosisReactive Oxygen Species-mediated Apoptosis
Garlic organosulfur components exhibit antitumor activity, but the molecular mechanisms underlying these effects have not been well characterized. We showed that Z-ajoene, a sulfur-rich compound purified from garlic, induced time- and dose-dependent apoptosis in HL-60 cells. This process implied the activation of caspase-3 and the cleavage of the antiapoptotic protein Bcl-2. The caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp-[OMe]-fluoromethylketone inhibited Bcl-2 cleavage and apoptosis induced by Z-ajoene. This effect was partially prevented by treatment of HL-60 cells with the antioxidant N-acetylcysteine. Hence, the transmission of apoptotic signal induced by Z-ajoene involved a reactive oxygen species-dependent pathway leading to caspase-dependent Bcl-2 cleavage.
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I. Vermes, C. Haanen, H M Steffens-Nakken et al. · Journal of Immunological Methods · 1995 · 5.4K citations · Full text
Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE
Yuri Lazebnik, Scott H. Kaufmann, Serge Desnoyers et al. · Nature · 1994 · 2.6K citations