Selective Fluorescence Detection of Small‐Molecule‐Binding Proteins by Using a Dual Photoaffinity Labeling System

Abstract

PHOTO OPPORTUNITY: We have developed a dual photoaffinity labeling system in which an active and an inactive probe bearing orthogonal detection groups are co-reacted in a single photoreaction. The approach allowed selective fluorescent detection of a model binding protein in cell lysate by either 1D or 2D electrophoresis.