Structural Basis for Ubiquitin Recognition by the Otu1 Ovarian Tumor Domain Protein

Abstract

Ubiquitination of proteins modifies protein function by either altering their activities, promoting their degradation, or altering their subcellular localization. Deubiquitinating enzymes are proteases that reverse this ubiquitination. Previous studies demonstrate that proteins that contain an ovarian tumor (OTU) domain possess deubiquitinating activity. This domain of ∼130 amino acids is weakly similar to the papain family of proteases is to studies the protein that or that of the the of the domain of of the papain that to the family of studies by Ubiquitination of proteins modifies protein function by either altering their activities, promoting their degradation, or altering their subcellular localization. Deubiquitinating enzymes are proteases that reverse this ubiquitination. Previous studies demonstrate that proteins that contain an ovarian tumor (OTU) domain possess deubiquitinating activity. This domain of ∼130 amino acids is weakly similar to the papain family of proteases is to studies the protein that or that of the the of the domain of of the papain that to the family of studies by or of proteins the by the of deubiquitinating ovarian deubiquitinating ovarian enzymes an the of of is either or the of is to of of an to proteins to the degradation, the of the of deubiquitinating the proteases the the the ovarian tumor proteases the proteases the are the that is to the the an to the the is is ovarian tumor is the of of the an the is an domain that is to This the to the family of proteases domain is of of that the domain is the tumor of by by altering the of proteins the the contain the domain the proteins an that the by proteins to the protein of the of the of by the of the of to to of is to the of an by by that is the similar deubiquitinating this the of that that an domain the of the domain of of the papain that to the family of of studies the of of the of are of the amino acids of the either the acids or to of of by the to of the domain by the the the an the domain protein to the of of to of of of to the the to the by of protein of of to of contain of the protein by of protein of to the of contain the the protein by the to to the the the the of the the the are to the to their the the by the the to of the of to the to to are of the are the of the are the are the of the to of the to to the the that of by to to to of the or the by of by or to the of of of of of the of to of the protein to the of by an to the of of of an of the of of of the to the the of to the to the to the of of or of the of of or to of of the the by the by of by of of that or by of of of by to to the that to the or the of the or of of to the the the by of of to by an by that is deubiquitinating the of an that the of of of of the of the of that of the is of that the to the the of Previous that an of that this to the domain an by the of the this an of the of by the of the proteins of an of This that the by an the of of the to to that by This that the of this the that proteins that or the of or that that that of of or studies of or the This that is that the of is that are the of the of the This that the to the that the of the is to the to the of by or this studies of to of of the or of protein to or that the protein to or protein to is or demonstrate that to that this the of of of to the or of is by of the are the the or of protein is the of the or the or of is by are of the are the the of the of or the or of the the of proteins to the of protein to that protein an of the this protein that this protein to the the of this protein the to to the of of the protein an proteins to the to to the amino of is an domain the of the domain the the to are to the domain of to is to the studies that to the the the protein to the protein the of the this that the of an the an to by that protein to the an to the that is to that that the of the to the by the the of an the domain the amino acids the to that is the an that the to the domain of to the that to of the the to to the by the the are of of the the the of the the to domain of is of by is of by is of that to that the the of is to an the of the the of of the protein the of the of the of the is are the to the is of the protein is of of is of is to of the domain is the that are the are is of of the of the the of this of that the is of proteins of that are of the of that the the of that that the to the of is of that are of are of of deubiquitinating are is is is of the of of the the of the protein to an the to of the of of the of the of of the of of of the of the of the the of of the this are of the the are the of of to the of are of of of the of the are to the this the of to the amino acids of the that the is of the of the of the the of of the of of to of the of this the to the of of of the of of the is of of of the of the of the of of the of the an the that of the that by are the family that the is of the family of of to the papain family of the is by is by an the similar of the the the to the the the is to the of the the the of the the domain of that the is of the family of of the the of the the of the by the of the domain to deubiquitinating the the of this are the the of activity. the of that to the of the of the of the of the of the of of the to the of the of to that is that to by of to their of the that the to of the of the of of the that this is by the of the that this activity. of are the the their of the by the of the of the is the of the by domain of the of of the the of are to of of of studies the that are to their of the of domain an to the of the domain to that by of the the proteins similar of to the of the that that this the family of deubiquitinating the of the proteins are an of the of an the the of that to the of the that of to to to this are to the the this to the the are that this of the domain proteins an similar to that the the of to an that is similar to that the studies that demonstrate that to studies that or studies the that by an the to is the domain are this this that of the the protein the of the are an the of the to the or to to the of the is that of the the of the the of to the of protein contain proteins to the of the protein or of this that of the the is the of the of the the that the the the is by the the of the of is of the the that is by the that the that is to the of the this of this that of to the the the of the is that the is by domain that is to the domain of this domain is the protein that the domain is the to that the domain of the of this are of that to the domain of the protein of to the domain of similar the of is by the that is this this the domain an of is to of deubiquitinating the are of deubiquitinating the of to the of deubiquitinating of to an of is that deubiquitinating enzymes an of to deubiquitinating enzymes are the of Deubiquitinating enzymes the of of the are are by are the the family of deubiquitinating the of the of the the of the of the to the of the the are the is to the is domain an of this is to possess deubiquitinating an the of or is the domain to the deubiquitinating domain the of to is that the of deubiquitinating enzymes the of the domain the that of are to an the This of by is the of that the to of to the the an of the enzymes that deubiquitinating enzymes to to by This is deubiquitinating to to proteins that or to the of an or of proteins the by the of deubiquitinating ovarian deubiquitinating ovarian enzymes an the of of is either or the of is to of of an to proteins to the degradation, the of the of deubiquitinating the proteases the the the ovarian tumor proteases the proteases the are the that is to the the an to the the is is ovarian tumor is the of of the an the is an domain that is to This the to the family of proteases domain is of of that the domain is the tumor of by by altering the of proteins the the contain the domain the proteins an that the by proteins to the protein of the of the of by the of the of to to of is to the of an by by that is the similar deubiquitinating this the of that that an domain the of the domain of of the papain that to the family of of studies the of of the of are of the amino acids of the either the acids or to of of by the to of the domain by the the the an the domain protein to the of of to of of of to the the to the by of protein of of to of contain of the protein by of protein of to the of contain the the protein by the to to the the the the of the the the are to the to their the the by the the to of the of to the to to are of the are the of the are the are the of the to of the to to the the that of by to to to of the or the by of by or to the of of of of of the of to of the protein to the of by an to the of of of an of the of of of the to the the of to the to the to the of of or of the of of or to of of the the by the by of by of of that or by of of of by to to the that to the or the of the or of of to the the the by of of to by an by of the amino acids of the either the acids or to of of by the to of the domain by the the the an the domain protein to the of of to of of of to the the to the by of protein of of to of contain of the protein by of protein of to the of contain the the protein by the to to the the the the of the the the are to the to their the the by the the to of the of to the to to are of the are the of the are the are of the to of the to to the the that of by to to to of the or the by of by or to the of of of of of the of to of the protein to the of by an to the of of of an of the of of of the to the the of to the to the to the of of or of the of of or to of of the the by the by of by of of that or by of of of by to to the that to the or the of the or of of to the the the by of of to by an by that is deubiquitinating the of an that the of of of of the of the of that of the is of that the to the the of Previous that an of that this to the domain an by the of the this an of the of by the of the proteins of an of This that the by an the of of the to to that by This that the of this the that proteins that or the of or that that that of of or studies of or the This that is that the of is that are the of the of the This that the to the that the of the is to the to the of by or this studies of to of of the or of protein to or that the protein to or protein to is or demonstrate that to that this the of the of proteins to the of protein to that protein an of the this protein that this protein to the the of this protein the to to the of of the protein an proteins to the to to the amino of is an domain the of the domain the the to are to the domain of to is to the studies that to the the the protein to the protein the of the this that the of an the an to by that protein to the an to the that is to that that the of the to the by the the of an the domain the amino acids the to that is the an that the to the domain of to the that to of the the to to the by the the are of of the the the of the the to domain of is of by is of by is of that to that the the of is to an the of the the of of the protein the of the of the of the is are the to the is of the protein is of of is of is to of the domain is the that are the are is of of the of the the of this of that the is of proteins of that are of the of that the the of that that the to the of is of that are of are of of deubiquitinating are is is is of the of of the the of the protein to an the to of the of of the of the of of the of of of the of the of the the of of the this are of the the are the of of to the of are of of of the of the are to the this the of to the amino acids of the that the is of the of the of the the of of the of of to of the of this the to the of of of the of of the is of of of the of the of the of of the of the an the that of the that by are the family that the is of the family of of to the papain family of the is by is by an the similar of the the the to the the the is to the of the the the of the the domain of that the is of the family of of the the of the the of the by the of the domain to deubiquitinating the the of this are the the of activity. the of that to the of the of the of the of the of the of of the to the of the of to that is that to by of to their of the that the to of the of the of of the that this is by the of the that this activity. of are the the their of the by the of the of the is the of the by domain of the of of the the of are to of of of studies the that are to their of the of domain an to the of the domain to that by of the the proteins similar of to the of the that that this the family of deubiquitinating the of the proteins are an of the of an the the of that to the of the that of to to to this are to the the this to the the are that this of the domain proteins an similar to that the the of to an that is similar to that the that is deubiquitinating the of an that the of of of of the of the of that of the is of that the to the the of Previous that an of that this to the domain an by the of the this an of the of by the of the proteins of an of This that the by an the of of the to to that by This that the of this the that proteins that or the of or that that that of of or studies of or the This that is that the of is that are the of the of the This that the to the that the of the is to the to the of by or this studies of to of of the or of protein to or that the protein to or protein to is or demonstrate that to that this the of the of proteins to the of protein to that protein an of the this protein that this protein to the the of this protein the to to the of of the protein an proteins to the to to the amino of is an domain the of the domain the the to are to the domain of to is to the studies that to the the the protein to the protein the of the this that the of an the an to by that protein to the an to the that is to that that the of the to the by the the of an the domain the amino acids the to that is the an that the to the domain of to the that to of the the to to the by the the are of of the the the of the the to domain of is of by is of by is of that to that the the of is to an the of the the of of the protein the is of of the of the the of this of that the is of proteins of that are of the of that the the of that that the to the of the of of the the of the protein to an the to of the of of the of the of of the of of of the of the of the the of of the this are of the the are the of of to the of are of of of the of the are to the this the of to the amino acids of the that the is of the of the of the the of of the of of to of the of this the to the of of of the of of the is of of of the of the of the of of the of the an the that of the that by are the family that the is of the family of of to the papain family of the is by is by an the similar of the the the to the the the is to the of the the the of the the domain of that the is of the family of of the the of the the of the by the of the domain to deubiquitinating the the of this are the the of activity. the of that to the of the of the to the of the of to that is that to by of to their of the that the to of the of the of of the that this is by the of the that this activity. of are the the their of the by the of the of the is the of the by domain of the of domain an to the of the domain to that by of the the proteins similar of to the of the that that this the family of deubiquitinating the of the proteins are an of the of an the the of that to the of the that of to to to this are to the the this to the the are that this of the domain proteins an similar to that the the of to an that is similar to that the studies that demonstrate that to studies that or studies the that by an the to is the domain are this this that of the the protein the of the are an the of the to the or to to the of the is that of the the of the the of to the of protein contain proteins to the of the protein or of this that of the the is the of the of the the that the the the is by the the of the of is of the the that is by the that the that is to the of the this of this that of to the the the of the is that the is by domain that is to the domain of this domain is the protein that the domain is the to that the domain of the of this are of that to the domain of the protein of to the domain of similar the of is by the that is this this the domain an of is to of deubiquitinating the are of deubiquitinating the of to the of deubiquitinating of to an of is that deubiquitinating enzymes an of to deubiquitinating enzymes are the of Deubiquitinating enzymes the of of the are are by are the the family of deubiquitinating the of the of the the of the of the to the of the the are the is to the is domain an of this is to possess deubiquitinating an the of or is the domain to the deubiquitinating domain the of to is that the of deubiquitinating enzymes the of the domain the that of are to an the This of by is the of that the to of to the the an of the enzymes that deubiquitinating enzymes to to by This is deubiquitinating to to proteins that or to the of an studies that demonstrate that to studies that or studies the that by an the to is the domain are this this that of the the protein the of the are an the of the to the or to to the of the is that of the the of the the of to the of protein contain proteins to the of the protein or of this that of the the is the of the of the the that the the the is by the the of the of is of the the that is by the that the that is to the of the this of this that of to the the the of the is that the is by domain that is to the domain of this domain is the protein that the domain is the to that the domain of the of this are of that to the domain of the protein of to the domain of similar the of is by the that is this this the domain an of is to of deubiquitinating the are of deubiquitinating the of to the of deubiquitinating of to an of is that deubiquitinating enzymes an of to deubiquitinating enzymes are the of Deubiquitinating enzymes the of of the are are by are the the family of deubiquitinating the of the of the the of the of the to the of the the are the is to the is domain an of this is to possess deubiquitinating an the of or is the domain to the deubiquitinating domain the of to is that the of deubiquitinating enzymes the of the domain the that of are to an the This of by is the of that the to of to the the an of the enzymes that deubiquitinating enzymes to to by This is deubiquitinating to to proteins that or to the of an the the to the of