Aestuariibaculum suncheonense gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from a tidal flat and emended descriptions of the genera Gaetbulibacter and Tamlana

Abstract

A Gram-staining-negative, yellow-pigmented, strictly aerobic bacterium, designated strain SC17(T), was isolated from sediment of a tidal flat of Suncheon bay in South Korea. Cells were halotolerant, catalase- and oxidase-positive and non-motile rods. Growth of strain SC17(T) was observed at 5-40 °C (optimum, 25-30 °C), at pH 6.0-8.5 (optimum, pH 7.0) and in the presence of 1-8 % (w/v) NaCl (optimum, 1-2 %). The major cellular fatty acids were iso-C(15 : 0), summed feature 3 (comprising C(16 : 1)ω7c and/or C(16 : 1)ω6c and/or iso-C(15 : 0) 2-OH), iso-C(17 : 0) 3-OH, iso-C(15 : 1) G and anteiso-C(15 : 0.) The polar lipid content consisted of phosphatidylethanolamine and unidentified amino lipids and lipids. The G+C content of the genomic DNA was 46.4 mol% and the only respiratory quinone detected was menaquinone-6 (MK-6). Phylogenetic inference based on 16S rRNA gene sequences showed that strain SC17(T) formed a distinct phyletic lineage within the family Flavobacteriaceae and was most closely related to members of the genera Gaetbulibacter and Tamlana with 95.0-95.8 % sequence similarity. On the basis of phenotypic and molecular features, strain SC17(T) represents a novel genus of the family Flavobacteriaceae, for which the name Aestuariibaculum suncheonense gen. nov., sp. nov. is proposed. The type strain is SC17(T) (= KACC 16186(T) = JCM 17789(T)). Emended descriptions of the genera Gaetbulibacter and Tamlana are also proposed.