EFFECT OF CYTOSOL ON LIPID PEROXIDATION IN FLOUNDER SARCOPLASMIC RETICULUM

Abstract

Studies were carried out to determine the effects of cytosol on sarcoplasmic reticulum lipid peroxidation. Upon addition of unfractionated cytosol to the lipid peroxidation system, inhibition was observed. Fractionation revealed that both the low molecular weight (LMW) cytosol (<10,000 MW) and high molecular weight (HMW) cytosol (>6,000–8,000 MW) fractions inhibited lipid peroxidation. It is suggested that either inorganic phosphate compounds or nucleotides may be responsible for the LMW cytosol's inhibitory characteristics. Addition of the HMW cytosol fraction to the system necessitated the presence of additional adenosine 5′-diphosphate (ADP) for optimal activity. ADP bound to the HMW cytosol fraction may be incapable of chelating the iron and preventing its precipitation as the ferric hydroxide.