Concepedia

Recombinant protein purification by self-cleaving aggregation tag

Wanyi Wu, Courtney Mee, Filomena Califano, Reza Banki, David W. Wood

Nature Protocols · 2006 · 74 citations · 19 references

DOI

Concepts

References

19

Purification of recombinant proteins by fusion with thermally-responsive polypeptides

Dan E. Meyer, Ashutosh Chilkoti · Nature Biotechnology · 1999 · 914 citations

, , +7

Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element

Shaorong Chong, Fana B. Mersha, Donald G. Comb et al. · Gene · 1997 · 606 citations

, , +16

Quantification of the Effects of Chain Length and Concentration on the Thermal Behavior of Elastin-like Polypeptides

Dan E. Meyer, Ashutosh Chilkoti · Biomacromolecules · 2004 · 567 citations

, , +17

A genetic system yields self-cleaving inteins for bioseparations

David W. Wood, Wei Wu, Georges Belfort et al. · Nature Biotechnology · 1999 · 275 citations

, , +6

Simple bioseparations using self-cleaving elastin-like polypeptide tags

Mahmoud Reza Banki, Liang Feng, David W. Wood · Nature Methods · 2005 · 238 citations

, , +14