Concepedia

Tag-based Purification

Tag-Based Purification is a methodological technique in enzyme purification that involves the selective isolation of recombinant enzymes through affinity binding mediated by a genetically fused molecular tag, distinguishing it from other purification approaches by its reliance on specific, engineered tags to enable highly specific capture and separation from complex biological mixtures.

345

Publications

17.3K

Citations

1.5K

Authors

475

Institutions

Publications per year

2017–2026

61

Parents

Publications

345

The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins

Thomas G.M. Schmidt, Arne Skerra · Nature Protocols · 2007 · 638 citations

, , +14

[16] Purification of proteins using polyhistidine affinity tags

Joshua A. Bornhorst, Joseph J. Falke · Methods in enzymology on CD-ROM/Methods in enzymology · 2000 · 556 citations

, , +12

Efficient biotinylation and single-step purification of tagged transcription factors in mammalian cells and transgenic mice

Ernie de Boer, Patrick Rodriguez, Edgar Bonte et al. · Proceedings of the National Academy of Sciences · 2003 · 413 citations · Full text

, , +13

Comparison of affinity tags for protein purification

Jordan J. Lichty, Joshua L. Malecki, Heather D. Agnew et al. · Protein Expression and Purification · 2005 · 408 citations

, , +14

A Tandem Affinity Tag for Two-step Purification under Fully Denaturing Conditions

Christian Tagwerker, Karin Flick, Meng Cui et al. · Molecular & Cellular Proteomics · 2006 · 337 citations · Full text

, , +16

1–5 of 345

Authors

1.5K

  1. DW
    David W. Wood

    Princeton University, United States

    7 publications · 506 citations

    7

    h-index

  2. TG
    Thomas G.M. Schmidt

    Technical University of Munich, Germany

    5 publications · 1.2K citations

    5

    h-index

  3. ZL
    Zhanglin Lin

    Tsinghua University, China

    5 publications · 152 citations

    5

    h-index

  4. YK
    Yukinari Kato

    Tohoku University, Japan

    4 publications · 164 citations

    4

    h-index

  5. MK
    Mika K. Kaneko

    Tohoku University, Japan

    4 publications · 164 citations

    4

    h-index

1–5 of 1.5K

Institutions

475

  1. Technical University of Munich

    Munich, Germany

    8 h-index

    19 publications

    1.6K citations

  2. Howard Hughes Medical Institute

    Chevy Chase, United States

    8 h-index

    14 publications

    1.6K citations

  3. Frederick National Laboratory for Cancer Research

    Frederick, United States

    7 h-index

    14 publications

    1.3K citations

  4. National Institutes of Health

    Bethesda, United States

    7 h-index

    76 publications

    2.6K citations

  5. University of Wisconsin–Madison

    Madison, United States

    7 h-index

    19 publications

    1.4K citations

  6. Tohoku University

    Sendai, Japan

    5 h-index

    21 publications

    818 citations

  7. The Ohio State University

    Columbus, United States

    5 h-index

    17 publications

    438 citations

  8. Chinese Academy of Sciences

    Beijing, China

    5 h-index

    26 publications

    857 citations

1–8 of 475

Venues

  1. Protein Expression and Purification

    52 publications · 2.3K citations

    23

    h-index

  2. Journal of Chromatography A

    14 publications · 658 citations

    13

    h-index

  3. PLoS ONE

    10 publications · 434 citations

    10

    h-index

  4. Methods in enzymology on CD-ROM/Methods in enzymology

    Book series · 9 publications · 1.1K citations

    9

    h-index

  5. Nature Protocols

    9 publications · 1K citations

    9

    h-index

1–5