Concepedia

Conformationally controlled high-affinity targeting of RNA or DNA by novel 2′-amino-DNA/LNA mixmers and pyrenyl-functionalized 2′-amino-DNA

Neerja Kalra, B. Ravindra Babu, Virinder S. Parmar, Jesper Wengel

Organic & Biomolecular Chemistry · 2004 · 35 citations · 12 references

DOI

Concepts

Abstract

9-Mer DNA sequences containing 2'-N-methyl-2'-N-(pyren-1-ylmethyl)-2'-amino-DNA monomers display significantly increased affinity towards DNA complements whereas the corresponding 2'-amino-DNA monomer has a detrimental effect on duplex stability. These effects are efficiently reversed by incorporation of four LNA nucleotides inducing a B-DNA to A-DNA conformational change.

References

12

LNA (Locked Nucleic Acids): Synthesis of the adenine, cytosine, guanine, 5-methylcytosine, thymine and uracil bicyclonucleoside monomers, oligomerisation, and unprecedented nucleic acid recognition

Alexei A. Koshkin, Sanjay K. Singh, Poul Nielsen et al. · Tetrahedron · 1998 · 1K citations

, , +16

Comparison of different antisense strategies in mammalian cells using locked nucleic acids, 2'-O-methyl RNA, phosphorothioates and small interfering RNA

Arnold Grünweller · Nucleic Acids Research · 2003 · 268 citations · Full text

, , +14

Locked Nucleic Acid (LNA) Recognition of RNA:  NMR Solution Structures of LNA:RNA Hybrids

Michael Petersen, Kent Bondensgaard, Jesper Wengel et al. · Journal of the American Chemical Society · 2002 · 260 citations

, , +17

Intercalating nucleic acids containing insertions of 1-O-(1-pyrenylmethyl)glycerol: stabilisation of dsDNA and discrimination of DNA over RNA

Ulf Bech Christensen · Nucleic Acids Research · 2002 · 137 citations

, , +17

2'-Pyrene modified oligonucleotide provides a highly sensitive fluorescent probe of RNA

Kazushige Yamana, Reiko Iwase, Shogo Furutani et al. · Nucleic Acids Research · 1999 · 127 citations · Full text

, , +14