Production of pig liver esterase in batch fermentation of E. coli Origami

A Thioredoxin Gene Fusion Expression System That Circumvents Inclusion Body Formation in the E. coli Cytoplasm

Edward R. LaVallie, Elizabeth Diblasio, Sharlotte Kovacic et al. · Nature Biotechnology · 1993 · 927 citations

Protein Expression, Natural Sciences, Gene Structure +8

The Role of the Thioredoxin and Glutaredoxin Pathways in Reducing Protein Disulfide Bonds in the Escherichia coliCytoplasm

William A. Prinz, Fredrik Åslund, Arne Holmgren et al. · Journal of Biological Chemistry · 1997 · 666 citations · Full text

Metabolic load and heterologous gene expression

Bernard R. Glick · Biotechnology Advances · 1995 · 655 citations

Systems Biology, Engineering, Metabolic Load +8

Efficient folding of proteins with multiple disulfide bonds in the <i>Escherichia coli</i> cytoplasm

Paul Bessette, Fredrik Åslund, Jon Beckwith et al. · Proceedings of the National Academy of Sciences · 1999 · 630 citations · Full text

Splicing by overlap extension by PCR using asymmetric amplification: an improved technique for the generation of hybrid proteins of immunological interest

Anthony Ν. Warrens, Michael D. Jones, Robert I. Lechler · Gene · 1997 · 285 citations

Rna Processing, Molecular Biological Method, Natural Sciences +13