Splicing by overlap extension by PCR using asymmetric amplification: an improved technique for the generation of hybrid proteins of immunological interest

Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension

Robert M. Horton, Henry D. Hunt, Steffan N. Ho et al. · Gene · 1989 · 3.1K citations

Life Sciences, Genetics, Molecular Biology +10

Three-dimensional structure of the human class II histocompatibility antigen HLA-DR1

Jerry H. Brown, Theodore S. Jardetzky, Joan C. Gorga et al. · Nature · 1993 · 2.4K citations

Life Sciences, Immunology, Molecular Biology +12

Filamentous coliphage M13 as a cloning vehicle: insertion of a HindII fragment of the lac regulatory region in M13 replicative form in vitro.

Joachim Messing, Bruno Gronenborn, Benno Müller‐Hill et al. · Proceedings of the National Academy of Sciences · 1977 · 708 citations · Full text

Life Sciences, Microbiology, Molecular Biology +16

A general method for introducing a series of mutations into cloned DNA using the polymerase chain reaction

Wataru Ito, Hiroshi Ishiguro, Yoshikazu Kurosawa · Gene · 1991 · 248 citations

Life Sciences, Molecular Biology, Molecular Genetics +10

The murine Eα immune response gene

Diane Mathis, Christophe Benoıst, V E Williams et al. · Cell · 1983 · 179 citations

Life Sciences, Immunology, Molecular Biology +12