Applied and Environmental Microbiology · 2009 · 43 citations · 17 references
Life SciencesBiochemistryBiotechnologyBiocatalysisCarbohydrate BiochemistryEnzymologyBiocatalyst EngineeringBiofuel ProductionBiomass ConversionEnzyme-substrate RecognitionGlycan DegradationIndustrial BiocatalysisCellulase EnzymesCellulase ProductionCellulosic BiofuelsHemicellulose DegradationLignocellulosic Biomass ConversionMultifunctional EnzymesPeptide LinkersXylan Degradation
Two highly active trifunctional hemicellulases were constructed by linking the catalytic portion of a xylanase with an arabinofuranosidase and a xylosidase, using either flexible peptide linkers or linkers containing a cellulose-binding domain. The multifunctional enzymes retain the parental enzyme properties and exhibit synergistic effects in hydrolysis of natural xylans and corn stover.
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