Optimization of Cellulose Enzyme in the Simultaneous Saccharification and Fermentation of Sugarcane Bagasse on the Second-generation Bioethanol Production Technology

Abstract

Sugarcane bagasse as raw material were treated on chemical delignification with NaOH and used for Simultaneous Saccharification and Fermentation (SSF). SSF was incubated during 5 days by adding Sacharomyches cereviceae, cellulose enzymes (Trichoderma reesei) and nutrients. The variable of this research was the cellulose enzyme variation of 10-60 fpu with 10 fpu on intervals. Optimal results were obtained on 60 fpu which producing ethanol content of 1.3544% or dry weight conversion of 21.3724 g/L with potential prediction shaped on logarithmic equation. In the advanced prediction can be obtained approximate optimal value of enzyme contents on 96.6802 fpu.