Journal of Agricultural and Food Chemistry · 2003 · 29 citations · 11 references
PharmacyBiochemistryPharmaceuticsDrug DeliveryFood ChemistryProtein EngineeringDisulfide Bond FormationDrug EncapsulationPeptide ChemistryPeptide DeliveryProtein DeliveryProteins In FoodsAmino AcidsBlood PressureNutraceutical EncapsulationPeptide Delivery SystemsPeptide EncapsulationProtein MicrocapsulesSynthetic Peptide CharacterizationSystolic Blood Pressure
Synthetic peptides were microencapsulated into liposomes, cycled with a disulfide bond or modified with d-phenylglycine (d-phg) at the N-terminal, and their antihypertensive effects as orally administered (0.18 mM/kg body weight) to spontaneously hypertensive rats (SHR) were measured. The microencapsulated Leu-Lys-Pro reduced significantly the systolic blood pressures of SHR by 45 mmHg and showed a prolonged duration, revealing the significant protective effect of encapsulation. d-phg-Leu-Arg-Pro showed a duration about 2 h shorter than that of the peptide without modification. In addition, cyclic Leu-Arg-Pro peptide with a disulfide bond between the N- and C-terminal amino acids reduced the systolic blood pressure of SHR by 35 mmHg and displayed a lengthy duration.
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