Expression of the Chlamydia trachomatis major outer membrane protein-encoding gene in Escherichia coli: role of the 3′ end in mRNA stability

DNA sequencing with chain-terminating inhibitors

Frederick Sanger, S. Nicklen, Alan Coulson · Proceedings of the National Academy of Sciences · 1977 · 69.1K citations · Full text

Dna, Engineering, Dna Analysis +20

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Harry Towbin, T. Staehelin, J. Gordon · Proceedings of the National Academy of Sciences · 1979 · 53.8K citations · Full text

Immunocytochemical Technique, Glycobiology, Polyacrylamide Gels +19

The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers

Jeffrey Vieira, Joachim Messing · Gene · 1982 · 6.1K citations

M13mp7-derived System, Dna Sequencing, Engineering +15

Screening λgt Recombinant Clones by Hybridization to Single Plaques in Situ

W. David Benton, Ronald W. Davis · Science · 1977 · 4.7K citations

Engineering, Genetics, λGt Recombinant Clones +22

Purification and partial characterization of the major outer membrane protein of Chlamydia trachomatis

Harlan D. Caldwell, J Kromhout, J Schachter · Infection and Immunity · 1981 · 1.1K citations · Full text

Biochemistry, Chlamydia Trachomatis, Natural Sciences +12