Publication | Closed Access
Screening λgt Recombinant Clones by Hybridization to Single Plaques in Situ
4.7K
Citations
16
References
1977
Year
EngineeringGeneticsλGt Recombinant ClonesSingle PlaquesBacteriophageMolecular BiologyMolecular GeneticsBioanalysisPhage BiologyMolecular DiagnosticsCloningGene TransferMolecular Biological MethodBiomolecular EngineeringPathogenesisBiotechnologyGenetic EngineeringSynthetic BiologyDirect MethodAgt Recombinant PhageNucleic Acid AmplificationMicrobiologyMedicineGenome EditingHigh-throughput Screening
A rapid, direct method for screening single plaques of Agt recombinant phage is described. The method allows at least 10(6) clones to be screened per day and simplifies physical containment of recombinants.
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