Concepedia

Screening λgt Recombinant Clones by Hybridization to Single Plaques in Situ

W. David Benton, Ronald W. Davis

Science · 1977 · 4.7K citations · 16 references

DOI

Concepts

Abstract

A rapid, direct method for screening single plaques of Agt recombinant phage is described. The method allows at least 10(6) clones to be screened per day and simplifies physical containment of recombinants.

References

16

Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.

Michael Grunstein, David S. Hogness · Proceedings of the National Academy of Sciences · 1975 · 3.5K citations · Full text

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A colony bank containing synthetic CoI EI hybrid plasmids representative of the entire E. coli genome

Louise Clarke, John Carbon · Cell · 1976 · 1.3K citations

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Hybridization of RNA to double-stranded DNA: formation of R-loops.

Michael R. Thomas, R. White, Ronald W. Davis · Proceedings of the National Academy of Sciences · 1976 · 481 citations · Full text

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Functional genetic expression of eukaryotic DNA in Escherichia coli.

Kevin Struhl, John Cameron, Ronald W. Davis · Proceedings of the National Academy of Sciences · 1976 · 261 citations · Full text

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Analysis of chromosomal integration and deletions of yeast plasmids

John Cameron, Peter Philippsen, Ronald W. Davis · Nucleic Acids Research · 1977 · 233 citations · Full text

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