Development of an enzymatic reaction device using magnetic bead-cluster handling

Abstract

We previously proposed a magnetic bead-cluster handling device for Micro-total analysis systems (Micro-TAS) and investigated its operation principle.The device does not need mechanical fluidic devices, such as pumps and valves, for handling solutions.We further developed the biochemical reaction unit chip, which is a key component in Micro-TAS, by applying a bead-cluster handling mechanism.We were able to do the enzymatic reaction by using hydrolysis between alkaline phosphatase and p-nitrophenyl phosphate.We also confirmed that the obtained calibration curves were linear during the enzymatic reaction.We had 70% reaction efficiency on the reaction chip by performing a comparative experiment.From these results, we concluded that our developed reaction chip is applicable to enzymatic immuno-assay systems.