Administration in suspension-form of n-alkane external markers for dry matter intake and diet selection studies

Abstract

SUMMARY Milled grass particles were coated with n-alkanes and suspended in a xanthan gum solution. Experiments snowed that grass particles could be coated uniformly with alkanes by means of a rotary evaporator. Further experiments showed that the grass particles, in stable xanthan gum suspension, could be accurately dispensed. The coefficients of variation of the alkane content per dose delivered by means of a dosing gun and syringe were 2·6 and 2·3%, respectively. There was no significant difference between the concentration of faecal alkanes derived from alkanes dosed in suspension-form and alkanes placed directly into the rumen.