Bioscience Biotechnology and Biochemistry · 2006 · 12 citations · 5 references
PharmacyBiochemistryMicrobiologyNatural Product BiosynthesisChiral ChromatographyChiral SeparationChromatographyLactic Acid BacteriaAbsolute Configuration AssignmentAbsolute Configuration DeterminationAnalytical Chiral ChromatographyChiral ColumnsChiral Intermediate ProductionChromatographic Chiral SeparationColumn ChromatographyEnantiomeric Purity Assessment
Optical resolution for (2S,3R) and (2R,3S)-hydroxycitric acid (HCA) enantiomers was developed using chiral column chromatography. HCA from Bacillus megaterium G45C and Streptomyces sp. U121, newly isolated in our previous study, was analyzed to determine the absolute configuration. These results indicate that both strains generate optically pure (2S,3R)-hibiscus type HCA enantiomer.
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