Bioscience Biotechnology and Biochemistry · 2009 · 43 citations · 8 references
Life SciencesBiochemistryMicrobiologyBiochemical EngineeringEnzymologyProtein EngineeringEnzyme FunctionEnzyme StabilityEnzyme StructureEnzyme Surface EngineeringProtein Engineering Of EnzymesStructural EnzymologyAlkaliphilic MicrobiologyPh Dependence Of CatalysisSalt Bridge FormationSalt Bridge InteractionsSalt Bridge NetworksSalt Bridge SystemsStructure-based Enzyme EngineeringXylanase Activity
Xylanase J (XynJ) from alkaliphilic Bacillus sp. 41M-1 is an alkaline xylanase. The crystal structure has been solved with XynJ. Improvement of the alkaliphily of XynJ was attempted by amino acid substitutions. Reinforcing the characteristic salt bridge in the catalytic cleft and introducing excess Arg residues on the protein surface shifted the optimum pH of the wild-type enzyme from 8.5 to 9.5.
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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
Ulrich K. Laemmli · Nature · 1970 · 251.6K citations
Site-directed mutagenesis by overlap extension using the polymerase chain reaction
Steffan N. Ho, Henry D. Hunt, Robert M. Horton et al. · Gene · 1989 · 7.5K citations
Ossi Turunen, Mika Vuorio, Fred Fenel et al. · Protein Engineering Design and Selection · 2002 · 147 citations · Full text