Science · 1987 · 150 citations · 23 references
Life SciencesCell BiologyImmunologyAdaptive ImmunityClinical ImmunologyImmunoregulationB Cell ActivationB Cell BiologyB-cell ResponsesHumoral ImmunityLymphocyte ActivationT Cell ActivationActivated B Cell ResponsesB Cell-mediated ImmunityB-cell Mediated ImmunityCompetitive Binding StudiesImmunoglobulin ResponsesImmunomodulatory Growth FactorsLymphotoxin Signaling
Two different assays for B cell growth factors (BCGF) and an antibody against lymphotoxin were used to show that the presence of lymphotoxin in conditioned media derived from normal activated T cells and in a partially purified BCGF accounts for a substantial portion of their B cell growth-promoting activity. A competitive binding assay confirmed the presence of significant amounts of lymphotoxin in the partially purified BCGF. Recombinant lymphotoxin enhanced the proliferation of activated B cells and augmented B cell proliferation and immunoglobulin secretion induced by interleukin-2.
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