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Critical factors in SEM 3D stereo microscopy
110
Citations
13
References
2008
Year
EngineeringMicroscopyMeasurementEducationBiomedical EngineeringReconstruction AccuracyElectron MicroscopyMicroscopy MethodCalibration3D ImagingComputational ImagingPhotometric StereoInstrumentationLight MicroscopyBiophysicsGeometric ModelingSem 3DDigital PhotogrammetryBiomedical ImagingElectron Microscope3D ScanningSurface TopographyInstrumental Variables
This work addresses dimensional measurements performed with the scanning electron microscope (SEM) using 3D reconstruction of surface topography through stereo-photogrammetry. The paper presents both theoretical and experimental investigations, on the effects of instrumental variables and measurement parameters on reconstruction accuracy. Investigations were performed on a novel sample, specifically developed and implemented for the tests. The description is based on the model function introduced by Piazzesi and adapted for eucentrically tilted stereopairs. Two main classes of influencing factors are recognized: the first one is related to the measurement operation and the instrument set-up; the second concerns the quality of scanned images and represents the major criticality in the application of SEMs for 3D characterizations.
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