Measurement of the reduced scattering coefficient of turbid media using single fiber reflectance spectroscopy: fiber diameter and phase function dependence

Abstract

This paper presents a relationship between the intensity collected by a single fiber reflectance device (R(SF)) and the fiber diameter (d(fib)) and the reduced scattering coefficient ( μs') and phase function (p(θ)) of a turbid medium. Monte Carlo simulations are used to identify and model a relationship between R(SF) and dimensionless scattering ( μs'dfib). For μs'dfib > 10 we find that R(SF) is insensitive to p(θ). A solid optical phantom is constructed with μs' ≈ 220 mm-1 and is used to convert R(SF) of any turbid medium to an absolute scale. This calibrated technique provides accurate estimates of μs' over a wide range ([0.05 - 8] mm(-1)) for a range of d(fib) ([0.2 - 1] mm).