Analytical Letters · 1987 · 137 citations · 10 references
Natural SciencesBiochemistryChemistryAnalytical ChemistryChromatographic AnalysisLipid BiochemistryChromatographyLipid ChemistryLiquid ChromatographyDetection Limit AssessmentHigh-performance Liquid ChromatographyInstrument Detection LimitLuminescence-based AssaysLuminescent Reporter SystemsMethod Detection LimitNormal-phase Chromatography
Abstract A chemiluminescence - high performance liquid chromatography (CL-HPLC) system was developed for the hydroperoxide-specific detection of phosphatidylcholine hydroperoxide. In this system, chemiluminescence detector was equipped with normal phase HPLC. Luminol-cytochrome c solution was used as the hydroperoxide-specific luminescent reagent. A detection limit of 7 nmol hydroperoxide-O2 of phosphatidylcholine hydroperoxide could be achieved. Key Words: HPLCchemiluminescencehydroperoxidephosphatidylcholine
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