Concepedia

Modified deoxyoligonucleotides stable to exonuclease degradation in serum

Jeng‐Pyng Shaw, Kenneth M. Kent, Jeff Bird, James A. Fishback, Brian C. Froehler

Nucleic Acids Research · 1991 · 369 citations · 13 references

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Abstract

Unmodified deoxyoligonucleotides are rapidly degraded in serum-containing medium. Utilizing internally labelled deoxyoligonucleotides the deoxyribonuclease profile for fetal calf serum and human serum was determined. It was found that the predominate nuclease activity in both systems was 3' exonuclease. Deoxyoligonucleotides are protected from exonuclease degradation in sera and cell media by simple terminal modifications that maintain high binding activity for the complementary DNA sequence.

References

13

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C.A. Stein, Kazuo Shinozuka, Jack S. Cohen · Nucleic Acids Research · 1988 · 584 citations · Full text

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Makoto Matsukura, Kazuo Shinozuka, Gerald Zon et al. · Proceedings of the National Academy of Sciences · 1987 · 535 citations · Full text

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Synthesis of DNA via deoxynudeoside H-phosphonate Intermediates

Brian C. Froehler, Peter G. Ng, Mark D. Matteucci · Nucleic Acids Research · 1986 · 345 citations · Full text

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Regulation of viral expression of human immunodeficiency virus in vitro by an antisense phosphorothioate oligodeoxynucleotide against rev (art/trs) in chronically infected cells.

Makoto Matsukura, Gerald Zon, Kazuo Shinozuka et al. · Proceedings of the National Academy of Sciences · 1989 · 263 citations · Full text

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Parallel Stranded DNA

Johan H. van de Sande, Niels B. Ramsing, Markus W. Germann et al. · Science · 1988 · 230 citations

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