Organic Process Research & Development · 2002 · 25 citations · 12 references
EngineeringCandida Antarctica LipaseAsymmetric CatalystsBiosynthesisBiochemical EngineeringAnalytical ChemistryThermodynamicsAnalytical BiotechnologyChemical BiotechnologyChromatographyThermoanalytical MethodRapid DeterminationBiochemistryBiocatalysisBiomolecular EngineeringThermographyPractical ApproachBiotechnologyMedicineDrug Analysis
Infrared thermography (IRT) is presented as a novel technique to screen a potentially large number of asymmetric catalysts or substrates in a high-throughput fashion. IRT was used as a simple, rapid, and practical approach for initial screening of the substrate specificity of Candida antarctica lipase. This was carried out using a 96-well microtitre plate format. Potential advantages and limitations of IRT for the enzymatic stereoselective acylation of primary and secondary alcohols of interest are discussed.
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