Biocatalysis and Biotransformation · 1998 · 679 citations · 140 references
Life SciencesBiochemistryBiotechnologyBiocatalysisBiochemical EngineeringEnzymologyBiocatalyst DiscoveryCatalytic MechanismsEnzyme DiscoveryEnzyme KineticsEnzyme-substrate RecognitionIndustrial BiocatalysisCrystal Structure PredictionLipase BiologyLipase CatalysisLipase DiscoveryLipasesMicrobial Lipase ActivitySubstrate Specificity Of LipasesYeast Lipase Activity
AbstractThe application of the B-component lipase from the yeast Candida antarctica in organic synthesis is reviewed. This enzyme has been found to be a particularly efficient and robust lipase catalyzing a surprising diversity of reactions including many different regio- and enantio-selec-tive syntheses. Furthermore, the C. antarctica B-lipase is an example of an enzyme for which its specificity has been predicted based on the crystal structure and modeling of the active site region. This prediction is compared to experimental observations and a very close correlation is found.Key Words: Candida antarctica B-lipasePredicted specificityRegio-selectivityEnantio-selectivityEstersAmidesPeroxycarboxylic acids
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The <i>α</i>/<i>β</i> hydrolase fold
David L. Ollis, Eong Cheah, Mirosław Cygler et al. · Protein Engineering Design and Selection · 1992 · 2.1K citations · Full text
Enzymatic catalysis in monophasic organic solvents
Jonathan S. Dordick · Enzyme and Microbial Technology · 1989 · 873 citations