Chemical Communications · 2013 · 17 citations · 19 references
EngineeringBiotransformationBiochemistryNatural SciencesBioconjugationBiotechnologyProtein PhosphorylationWittig OlefinationBiomolecular InteractionProtein EngineeringChemical BiologyFluorescence TaggingStabilized Phosphorus YlidesBioconjugation ReactionBio-orthogonal ChemistryBiomolecular Engineering
Stabilized Wittig olefination holds great potential as a bioconjugation reaction. We demonstrate that the reaction of stabilized phosphorus ylides (or phosphonium salts) with aryl aldehydes is sufficiently robust to be used for live cell affinity isolation and fluorescence tagging of a protein, FKBP12.
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Copper-free click chemistry in living animals
Pamela V. Chang, Jennifer A. Prescher, Ellen M. Sletten et al. · Proceedings of the National Academy of Sciences · 2010 · 637 citations · Full text
Bioconjugation with Strained Alkenes and Alkynes
Marjoke F. Debets, Sander S. van Berkel, Jan Dommerholt et al. · Accounts of Chemical Research · 2011 · 548 citations