Detection and Quantitation of Succinimide in Intact Protein via Hydrazine Trapping and Chemical Derivatization

Deamidation, isomerization, and racemization at asparaginyl and aspartyl residues in peptides. Succinimide-linked reactions that contribute to protein degradation.

Terrence L. Geiger, Steven Clarke · Journal of Biological Chemistry · 1987 · 1.3K citations · Full text

Life Sciences, Biochemistry, Molecular Biology +16

Succinimide Formation from Aspartyl and Asparaginyl Peptides as a Model for the Spontaneous Degradation of Proteins

Robert C. Stephenson, Steven Clarke · Journal of Biological Chemistry · 1989 · 518 citations · Full text

Life Sciences, Biochemistry, Post-translational Modification +14

Identification of multiple sources of charge heterogeneity in a recombinant antibody

Reed J. Harris, Bruce Kabakoff, Frank Macchi et al. · Journal of Chromatography B Biomedical Sciences and Applications · 2001 · 503 citations

Pharmacy, Biochemistry, Molecular Biology +16

Chemical Pathways of Peptide Degradation. II. Kinetics of Deamidation of an Asparaginyl Residue in a Model Hexapeptide

Kamlesh C. Patel, Ronald T. Borchardt · Pharmaceutical Research · 1990 · 279 citations

Life Sciences, Biochemistry, Molecular Biology +17

In Vivo Deamidation Characterization of Monoclonal Antibody by LC/MS/MS

Lihua Huang, Jirong Lu, Victor J. Wroblewski et al. · Analytical Chemistry · 2005 · 265 citations

Pharmacy, Pharmaceutics, Pharmacology +17