Transgenic Apple (<i>Malus</i> × <i>domestica</i>) Shoot Showing Low Browning Potential

Abstract

Transgenic apple shoots were prepared from leaf disks by using Agrobacterium tumefaciens carrying the kanamycin (KM) resistance gene and antisense polyphenol oxidase (PPO) DNA. Four transgenic apple lines that grew on the medium containing 50 μg/mL KM were obtained. They contained the KM resistance gene and grew stably on the medium for >3 years. Two transgenic shoot lines containing antisense PPO DNA in which PPO activity was repressed showed a lower browning potential than a control shoot. Keywords: Apple (Malus × domestica); enzymatic browning; polyphenol oxidase; antisense; transgenic plant; Agrobacterium transformation