Halophilic β-lactamase as a new solubility- and folding-enhancing tag protein: production of native human interleukin 1α and human neutrophil α-defensin

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

Ulrich K. Laemmli · Nature · 1970 · 251.6K citations

Life Sciences, Microbiology, Molecular Biology +14

Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa

Hermann Schägger, Gebhard von Jagow · Analytical Biochemistry · 1987 · 11.5K citations

Life Sciences, Biochemistry, Molecular Biology +10

<i>Escherichia coli</i> maltose‐binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused

Rachel B. Kapust, David S. Waugh · Protein Science · 1999 · 964 citations · Full text

Life Sciences, Biochemistry, Molecular Biology +16

Ultrasensitive assays for endogenous antimicrobial polypeptides

Robert I. Lehrer, Michael A. Rosenman, Sylvia S.S.L. Harwig et al. · Journal of Immunological Methods · 1991 · 669 citations

Life Sciences, Microbiology, Biosensors +9

Human interleukin 1 is a cytocidal factor for several tumor cell lines.

Kikuo Onozaki, Koji Matsushima, B B Aggarwal et al. · The Journal of Immunology · 1985 · 377 citations

Life Sciences, Cell Biology, Immunology +9