Effect of water on the enzymatic synthesis of vinyl sugar ester in hydrophilic organic solvent

Abstract

The transesterification of divinyl adipate with glucose in N,N-dimethylformamide (DMF) catalyzed by alkaline protease from Streptomyces sp. and Bacillus subtilis at various water contents were examined. The enzymatic reaction by the Bacillus protease was carried out effectively in the presence of more than 2% of water, and a maximum reaction rate was observed at a water content of 20%. On the other hand, the reaction by the Streptomyces protease proceeded more effectively without the addition of water than in the presence of water. These results suggest that optimum water content is different, depending on the kind of enzyme protein used. The effect of water on the enzymatic synthesis of vinyl glucose ester catalyzed by protease from Bacillus subtilis (B) in DMF. The reaction conditions are outlined in the Experimental Part.