The lipids of slugs and snails: Evolution, diet and biosynthesis

Abstract

There is a considerable gap in current knowledge of the lipid composition of snails and slugs, both of which belong to the phylum Mollusca. We have therefore analyzed the sterol and fatty acid compositions of three species of slugs and three species of snails. The sterols of slugs included eight different sterols: cholesterol contributed 76-85% of the total sterols, brassicasterol accounted for 4-13%; other sterols we identified were lathosterol, 24-methylene cholesterol, campesterol, stigmasterol, sitosterol and sitostanol. In contrast, snails contained two additional sterols, desmosterol and cholestanol. Of the polyunsaturated fatty acids in slugs, linoleic (18:2n-6) and arachidonic acids (20:4n-6) were the major n-6 fatty acids, while linoleic (18:3n-3) and eicosapentaenoic acids (20:5n-3) were the predominant n-3 fatty acids. Docosahexaenoic acid (22:6n-3), the end product in the n-3 fatty acid synthetic pathway and an important membrane fatty acid of mammals, fish and birds, was absent in both slugs and snails. However, the analogous product of n-6 fatty acid synthesis, 22:5n-6, was found in both snails and slugs. This raises speculation about preference for n-6 fatty acid synthesis in these species. Our data show the unique sterol and fatty acid compositions of slugs and snails, as well as similarities and differences in sterol composition between the two. The results between the two land mollusks are contrasted with those of marine mollusks, such as oysters, clams and scallops.