Publication | Closed Access
Increasing the stability of an enzyme toward hostile organic solvents by directed evolution based on iterative saturation mutagenesis using the B-FIT method
162
Citations
9
References
2010
Year
EngineeringBacteriologyMolecular BiologyBacillus SubtilisB-fit MethodOrganic SolventsBioenergeticsBiochemical EngineeringStructure-function Enzyme KineticsChemical BiotechnologyBiochemistryBiocatalysisDirected EvolutionMolecular MicrobiologyBiomolecular EngineeringHostile Organic SolventsNatural SciencesEnzyme CatalysisBiotechnologySynthetic BiologyProtein EngineeringMicrobiologyIterative Saturation Mutagenesis
Mutants of the lipase from Bacillus subtilis, previously engineered for enhanced thermostability using directed evolution based on the B-FIT method, show significantly increased tolerance to hostile organic solvents.
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