FEBS Letters · 2006 · 28 citations · 26 references
Life SciencesHematologyMolecular BiologyCell SignalingHormone BiochemistrySignal TransductionAdipose TissueCytokine BiologyCytokine SignalingHormone Binding ProteinsHormone ReceptorsHormone SignalingCytokine Signaling In HematopoiesisHematopoietic SignalingHormone Receptor Binding ProteinsLeptin SignalingPeptide Hormone Binding ProteinsPhospholipase C Gamma SignalingPhospholipase C SignalingProtein Hormones
The adipocyte-secreted hormone leptin participates in the regulation of hematopoiesis and enhances proliferation of hematopoietic cells. We used an adaptation of the MAPPIT mammalian two-hybrid method to study leptin signalling in a hematopoietic setting. We confirmed the known interactions of suppressor of cytokine signalling 3 (SOCS3) and STAT5 with the Y985 and Y1077 motifs of the leptin receptor, respectively. We also provide evidence for novel interactions at the Y1077 motif, including phospholipase C gamma and several members of the SOCS protein family, further underscoring the important role of the Y1077 motif in leptin signalling.
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