Concepedia

A single blastocyst assay optimized for detecting CRISPR/Cas9 system-induced indel mutations in mice

Takayuki Sakurai, Satoshi Watanabe, Akiko Kamiyoshi, Masahiro Sato, Takayuki Shindo

BMC Biotechnology · 2014 · 94 citations · 17 references

Open access

Abstract

The single blastocyst-based assay was useful for determining whether CRISPR/Cas9-mediated genome editing worked in murine embryos.

References

17

A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity

Martin Jínek, Krzysztof Chylinski, Ines Fonfara et al. · Science · 2012 · 16.7K citations · Full text

Crispr Rnas, Adaptive Bacterial Immunity, Crispr-associated Protein Cas9 +15

Multiplex Genome Engineering Using CRISPR/Cas Systems

Le Cong, F. Ann Ran, David Cox et al. · Science · 2013 · 15.4K citations

Single Crispr Array, Engineering, Short Rnas +12

RNA-Guided Human Genome Engineering via Cas9

Prashant Mali, Luhan Yang, Kevin M. Esvelt et al. · Science · 2013 · 9.2K citations · Full text

Engineering, Genetics, Molecular Biology +16

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

Haoyi Wang, Hui Yang, Chikdu Shivalila et al. · Cell · 2013 · 3.5K citations · Full text

Off-target Effect, Engineering, Genome Surgery +12

One-Step Generation of Mice Carrying Reporter and Conditional Alleles by CRISPR/Cas-Mediated Genome Engineering

Hui Yang, Haoyi Wang, Chikdu Shivalila et al. · Cell · 2013 · 1.6K citations · Full text

Knockout Mouse, Engineering, Genome Surgery +13