Concepedia

Site-specific protein modification using immobilized sortase in batch and continuous-flow systems

Martin D. Witte, Tongfei Wu, Carla P. Guimarães, Christopher S. Theile, Annet E. M. Blom, Jessica R. Ingram, Zeyang Li, Lenka Kundrat, Shalom D. Goldberg, Hidde L. Ploegh

Nature Protocols · 2015 · 77 citations · 20 references

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References

20

A general strategy for the evolution of bond-forming enzymes using yeast display

Irwin Chen, Brent M. Dorr, David R. Liu · Proceedings of the National Academy of Sciences · 2011 · 590 citations · Full text

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Sortagging: a versatile method for protein labeling

Maximilian W. Popp, John M. Antos, Gijsbert M. Grotenbreg et al. · Nature Chemical Biology · 2007 · 543 citations

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Sortase-Mediated Protein Ligation:  A New Method for Protein Engineering

Hongyuan Mao, Scott A. Hart, Amy Schink et al. · Journal of the American Chemical Society · 2004 · 536 citations

, , +14

Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions

Carla P. Guimarães, Martin D. Witte, Christopher S. Theile et al. · Nature Protocols · 2013 · 352 citations · Full text

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Site-specific N-terminal labeling of proteins using sortase-mediated reactions

Christopher S. Theile, Martin D. Witte, Annet E. M. Blom et al. · Nature Protocols · 2013 · 281 citations · Full text

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