Practical Methods for Chemical Inactivation of Creutzfeldt‐Jakob Disease Pathogen

Abstract

Chemical inactivation of pathogen of Creutzfeldt-Jakob disease (CJD) was examined using the mouse-adapted CJD strain. A high concentration of formic acid, guanidine compounds, trichloroacetate and phenol prevented CJD transmission. NaOH between 0.25 and 2 N lengthened the incubation periods. Sodium dodecyl sulfate (SDS) in a concentration between 1 and 3% did not alter incubation at room temperature but did completely block the transmission after boiling for 3 min in 3% SDS. This method is recommended for practical disinfection.