Detection and quantification of Brettanomyces bruxellensis and 'ropy'Pediococcus damnosus strains in wine by real-time polymerase chain reaction

Abstract

The real-time PCR-based method described in this study provides a new tool for monitoring spoilage micro-organisms in wine. Time-consuming culture and colony isolation steps are no longer needed, so winemakers can intervene before spoilage occurs.