ChemBioChem · 2010 · 18 citations · 17 references
Life SciencesBiochemistryMolecular BiologyBiocatalysisEnzymologyMetagenomicsBiocatalyst DiscoveryCatalytic MechanismsEnzymatic CatalysisEnzyme DiscoveryEnzyme-substrate RecognitionHydrolase CatalysisCatalytic PromiscuityDehalogenasesEsterase-mediated HydrolysisHydrolase DiscoveryMetagenomic Enzyme DiscoveryMetagenomic Enzyme EngineeringPromiscuous Catalysis
Promiscuous proteins: The identification and characterization of a wild-type promiscuous hydrolase that efficiently catalyzes the hydrolysis of ester bonds and haloacids in a single protein has been reported. This study has demonstrated the utility of metagenomics to access novel catalytic activities.
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The <i>α</i>/<i>β</i> hydrolase fold
David L. Ollis, Eong Cheah, Mirosław Cygler et al. · Protein Engineering Design and Selection · 1992 · 2.1K citations · Full text
The 'evolvability' of promiscuous protein functions
Amir Aharoni, Leonid Gaidukov, Olga Khersonsky et al. · Nature Genetics · 2004 · 832 citations
A. Maxwell Burroughs, Karen N. Allen, Debra Dunaway‐Mariano et al. · Journal of Molecular Biology · 2006 · 458 citations