High-level production of human growth hormone in Escherichia coli by a simple recombinant process

[6] Use of T7 RNA polymerase to direct expression of cloned genes

F. William Studier, Alan H. Rosenberg, John J. Dunn et al. · Methods in enzymology on CD-ROM/Methods in enzymology · 1990 · 6.6K citations · Full text

Life Sciences, Molecular Biology, Molecular Genetics +10

Human Growth Hormone and Extracellular Domain of Its Receptor: Crystal Structure of the Complex

Abraham M. de Vos, Mark Ultsch, Anthony A. Kossiakoff · Science · 1992 · 2.2K citations

Life Sciences, Biochemistry, Molecular Biology +17

Human tumour necrosis factor: precursor structure, expression and homology to lymphotoxin

Diane Pennica, Glenn E. Nedwin, Joel S. Hayflick et al. · Nature · 1984 · 1.7K citations

Life Sciences, Immunology, Immunopathology +9

A Thioredoxin Gene Fusion Expression System That Circumvents Inclusion Body Formation in the E. coli Cytoplasm

Edward R. LaVallie, Elizabeth Diblasio, Sharlotte Kovacic et al. · Nature Biotechnology · 1993 · 931 citations

Life Sciences, Genetics, Molecular Biology +11

Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein

Chu di Guana, Ping Lib, Paul D. Riggsa et al. · Gene · 1988 · 675 citations

Life Sciences, Biotechnology, Molecular Biology +9