Food Science and Technology Research · 2014 · 21 citations · 20 references
AgricultureFood ScienceMicrobiologyBiosensorsFood MicrobiologyFood SafetyFoodborne Pathogen DetectionFoodborne PathogensMicrobial Food SafetyMicrobiological Food SafetyPathogen DetectionPathogen Detection In FoodsFood Safety BiosensorsFoodborne Bacterial PathogensPlasmonic BiosensorsPolyclonal AntibodiesRapid Detection AssaysSurface Plasmon Resonance BiosensingSurface Plasmon Resonance BiosensorsSurface Plasmon Resonance Sensors
Surface plasmon resonance (SPR) biosensors were used to develop a rapid and simultaneous detection method for three important foodborne pathogens, i.e., Escherichia coli O157:H7 (O157), Salmonella Enteritidis (SE), and Listeria monocytogenes (LM). Bacterial homogenates prepared by sonication from bacterial suspensions at various cell concentrations were analyzed using SPR biosensors and sensor chips with polyclonal antibodies specific to each of the target pathogens. The precipitates from the homogenates were demonstrated to be suitable for specific and simultaneous detection of O157, SE, and LM. By using the precipitates and a custom-built multichannel SPR biosensor, the lower detection limit for O157, SE, and LM was determined to be 0.6 × 106, 1.8 × 106, and 0.7 × 107 CFU/mL, respectively, in the presence of non-target pathogens at concentrations of 105 to 108 CFU/mL.
20