Plasma Processes and Polymers · 2015 · 53 citations · 42 references
Natural SciencesBioengineeringMaterials ScienceBiomaterialsProtein BiochemistryProtein EngineeringBiomaterial CharacterizationCoating TechnologyCoatingsMicrobial BiopolymersNanocompositesSurface CoatingsAtmospheric Pressure Plasma TreatmentBiomaterial CoatingsLysozyme ActivityPlasma DepositionPlasma-enhanced DepositionProtein Binding DomainsProtein CoatingsProtein-based Materials
Bio-composite coatings, consisting of an organic matrix embedding a bioactive molecule, have been deposited by means of atomizer-assisted atmospheric pressure plasma. Ethylene was chosen as the precursor of the matrix, while the atomizer was fed with a water solution of lysozyme. Coatings chemical composition was investigated by XPS, FTIR and MALDI-TOF spectroscopies, and it has been proved that the one-step inclusion of protein domains in the composite coatings is successful and lysozyme chemical structure is only slightly altered. The amount of embedded lysozyme is as high as 14 μg/cm2 as evaluated from water release test. Finally, the activity of the plasma-embedded protein is close to that of pure lysozyme as verified against Micrococcus lysodeikticus ATCC 4698 through an agar plate diffusion test.
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Electrical detection of single viruses
Fernando Patolsky, Gengfeng Zheng, Oliver Hayden et al. · Proceedings of the National Academy of Sciences · 2004 · 1.3K citations · Full text
Catherine Picart, Corinne Taddéi, Youssef Haïkel et al. · Antimicrobial Agents and Chemotherapy · 2004 · 204 citations · Full text