Targeted transgene insertion into the <i>AAVS1</i> locus driven by baculoviral vector‐mediated zinc finger nuclease expression in human‐induced pluripotent stem cells

Abstract

Given high targeting efficiency, stability in expression of inserted transgene and flexibility in transgene exchange, the approach reported in the present study holds potential for generating genetically-modified human pluripotent stem cells suitable for developmental biology research, drug development, regenerative medicine and gene therapy.