Phage Display

Phage Display is a molecular laboratory technique for protein engineering and directed evolution in which genes encoding variant peptides or proteins are fused to bacteriophage coat protein genes so that the encoded products are displayed on the phage surface while their coding sequences remain inside the virion, thereby physically linking genotype and phenotype to enable iterative in vitro selection of variants with desired binding or functional properties.

2.6K

Publications

146.9K

Citations

11.1K

Authors

2.3K

Institutions

Publications per year

2017–2026

399

Parents

Antibody EngineeringDirected Evolution

Children

Antibody Fragment DisplayAntigen-specific Antibody DiscoveryBinder Specificity OptimizationBiopanningConsensus Sequence AnalysisDirected Evolution By Phage DisplayDisplay Host EngineeringFab DisplayLibrary Diversity GenerationLibrary Enrichment Analysis

Publications

2.6K

Filamentous Fusion Phage: Novel Expression Vectors That Display Cloned Antigens on the Virion Surface

George P. Smith · Science · 1985 · 4.1K citations

Life Sciences, Biotechnology, Molecular Biology +16

Phage antibodies: filamentous phage displaying antibody variable domains

John McCafferty, Andrew D. Griffiths, Greg Winter et al. · Nature · 1990 · 2.4K citations

Pharmacy, Immunology, Molecular Biology +16

PHAST: A Fast Phage Search Tool

You Zhou, Yong Liang, Karlene H. Lynch et al. · Nucleic Acids Research · 2011 · 2.1K citations · Full text

Life Sciences, Bioinformatics, Microbiology +17

Searching for Peptide Ligands with an Epitope Library

Jamie K. Scott, George P. Smith · Science · 1990 · 2.1K citations

Life Sciences, Biochemistry, Molecular Biology +17

Yeast surface display for screening combinatorial polypeptide libraries

Eric T. Boder, K. Dane Wittrup · Nature Biotechnology · 1997 · 1.8K citations

Engineering, Biochemistry, Biotechnology +13

1–5 of 2.6K

Authors

11.1K

VA
Valery A. Petrenko
Auburn University, United States
30 publications · 2K citations
24
h-index
SS
Sachdev S. Sidhu
University of Toronto, Canada
30 publications · 2.8K citations
23
h-index
GW
Greg Winter
MRC Laboratory of Molecular Biology, United Kingdom
25 publications · 8.6K citations
23
h-index
JD
James D. Marks
University of California, San Francisco, United States
27 publications · 2.6K citations
22
h-index
AB
Andrew Bradbury
Los Alamos National Laboratory, United States
24 publications · 1.3K citations
21
h-index

1–5 of 11.1K

Institutions

2.3K

1–8 of 2.3K

Venues

Journal of Molecular Biology
JMB · 104 publications · 9.7K citations
54
h-index
Proceedings of the National Academy of Sciences
PNAS · 53 publications · 9K citations
49
h-index
Journal of Immunological Methods
110 publications · 4.8K citations
41
h-index
Journal of Biological Chemistry
JBC · 67 publications · 5.6K citations
41
h-index
Gene
54 publications · 3.9K citations
34
h-index

1–5