Concepedia

Membrane Protein Expression Optimization

Membrane Protein Expression Optimization is a methodological framework in molecular biotechnology that systematically enhances the yield and functionality of membrane proteins in heterologous expression systems through strategic modulation of genetic, cellular, and environmental variables.

85

Publications

5.1K

Citations

420

Authors

166

Institutions

Publications per year

2017–2026

16

Parents

Publications

85

Tuning <i>Escherichia coli</i> for membrane protein overexpression

Samuel Wagner, Mirjam Klepsch, Susan Schlegel et al. · Proceedings of the National Academy of Sciences · 2008 · 453 citations · Full text

, , +16

Optimization of membrane protein overexpression and purification using GFP fusions

David Drew, Mirjam Lerch, Edmund R.S. Kunji et al. · Nature Methods · 2006 · 340 citations

, , +14

GFP-based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae

David Drew, Simon Newstead, Yo Sonoda et al. · Nature Protocols · 2008 · 322 citations · Full text

, , +15

Preparative scale expression of membrane proteins in Escherichia coli-based continuous exchange cell-free systems

Dániel Schwarz, Friederike Junge, Florian Durst et al. · Nature Protocols · 2007 · 270 citations

, , +10

High-throughput fluorescent-based optimization of eukaryotic membrane protein overexpression and purification in <i>Saccharomyces cerevisiae</i>

Simon Newstead, Hyun Kim, Gunnar von Heijne et al. · Proceedings of the National Academy of Sciences · 2007 · 230 citations · Full text

, , +17

1–5 of 85

Authors

420

  1. DD
    David Drew

    Stockholm University, Sweden

    8 publications · 1.2K citations

    8

    h-index

  2. JD
    Jan‐Willem De Gier

    Stockholm University, Sweden

    6 publications · 1K citations

    6

    h-index

  3. SI
    So Iwata

    Kyoto University, Japan

    5 publications · 617 citations

    5

    h-index

  4. ER
    Edmund R.S. Kunji

    Medical Research Council, United Kingdom

    4 publications · 478 citations

    4

    h-index

  5. DJ
    Dirk Jan Slotboom

    University of Groningen, Netherlands

    4 publications · 723 citations

    4

    h-index

1–5 of 420

Institutions

166

  1. Stockholm University

    Stockholm, Sweden

    13 h-index

    57 publications

    8.6K citations

  2. Imperial College London

    London, United Kingdom

    9 h-index

    22 publications

    2.6K citations

  3. Centre National de la Recherche Scientifique

    Paris, France

    7 h-index

    37 publications

    2.8K citations

  4. University of Groningen

    Groningen, The Netherlands

    6 h-index

    13 publications

    1.9K citations

  5. Goethe University Frankfurt

    Frankfurt am Main, Germany

    5 h-index

    24 publications

    3.3K citations

  6. Institute of Molecular Life Sciences

    Budapest, Hungary

    5 h-index

    6 publications

    1.1K citations

  7. Université Claude Bernard Lyon 1

    Villeurbanne, France

    4 h-index

    9 publications

    270 citations

  8. University of Virginia

    Charlottesville, United States

    3 h-index

    6 publications

    605 citations

1–8 of 166

Venues

  1. Protein Expression and Purification

    11 publications · 490 citations

    11

    h-index

  2. Protein Science

    PS · 8 publications · 390 citations

    8

    h-index

  3. Methods in molecular biology

    Book series · 7 publications · 176 citations

    7

    h-index

  4. PLoS ONE

    4 publications · 366 citations

    4

    h-index

  5. Journal of Biological Chemistry

    JBC · 4 publications · 156 citations

    4

    h-index

1–5