Jan‐Willem De Gier

Tuning <i>Escherichia coli</i> for membrane protein overexpression

Samuel Wagner, Mirjam Klepsch, Susan Schlegel et al. · Proceedings of the National Academy of Sciences · 2008 · 453 citations · Full text

Life Sciences, Biotechnology, Molecular Biology +16

Consequences of Membrane Protein Overexpression in Escherichia coli

Samuel Wagner, Louise Baars, A. Jimmy Ytterberg et al. · Molecular & Cellular Proteomics · 2007 · 378 citations · Full text

Life Sciences, Microbiology, Molecular Biology +17

Optimization of membrane protein overexpression and purification using GFP fusions

David Drew, Mirjam Lerch, Edmund R.S. Kunji et al. · Nature Methods · 2006 · 340 citations

Engineering, Biochemistry, Molecular Biology +14

Competition between Sec‐ and TAT‐dependent protein translocation in Escherichia coli

Susana Cristóbal, Jan‐Willem De Gier, Henrik Nielsen et al. · The EMBO Journal · 1999 · 276 citations

Life Sciences, Biochemistry, Molecular Biology +7

Rapid topology mapping of <i>Escherichia coli</i> inner-membrane proteins by prediction and PhoA/GFP fusion analysis

David Drew, Dan Sjöstrand, Johan Nilsson et al. · Proceedings of the National Academy of Sciences · 2002 · 200 citations

Life Sciences, Biochemistry, Microbiology +15