Jan‐Willem De Gier

Tuning <i>Escherichia coli</i> for membrane protein overexpression

Samuel Wagner, Mirjam Klepsch, Susan Schlegel et al. · Proceedings of the National Academy of Sciences · 2008 · 444 citations · Full text

Protein Expression, Medicine, Different Strains +13

Consequences of Membrane Protein Overexpression in Escherichia coli

Samuel Wagner, Louise Baars, A. Jimmy Ytterberg et al. · Molecular & Cellular Proteomics · 2007 · 369 citations · Full text

Optimization of membrane protein overexpression and purification using GFP fusions

David Drew, Mirjam Lerch, Edmund R.S. Kunji et al. · Nature Methods · 2006 · 337 citations

Membrane Fusion, Biochemistry, Natural Sciences +9

Rapid topology mapping of <i>Escherichia coli</i> inner-membrane proteins by prediction and PhoA/GFP fusion analysis

David Drew, Dan Sjöstrand, Johan Nilsson et al. · Proceedings of the National Academy of Sciences · 2002 · 199 citations

Optimizing Membrane Protein Overexpression in the Escherichia coli strain Lemo21(DE3)

Susan Schlegel, John Löfblom, Chiara Lee et al. · Journal of Molecular Biology · 2012 · 163 citations

Medicine, Natural Sciences, Protein Biosynthesis +8