Concepedia

Focused Mutagenesis

Focused Mutagenesis is a site-directed mutagenesis strategy that belongs to the broader class of targeted genetic modification techniques, distinguished by its precise alteration of specific nucleotide sequences within a gene to investigate structure-function relationships or engineer desired phenotypic traits.

40

Publications

2.6K

Citations

249

Authors

97

Institutions

Publications per year

2017–2026

16

Parents

Publications

40

A systematic, genome-wide, phenotype-driven mutagenesis programme for gene function studies in the mouse

Patrick M. Nolan, Jo Peters, Mark Strivens et al. · Nature Genetics · 2000 · 658 citations

, , +14

Stereodivergent Protein Engineering of a Lipase To Access All Possible Stereoisomers of Chiral Esters with Two Stereocenters

Jian Xu, Yixin Cen, Warispreet Singh et al. · Journal of the American Chemical Society · 2019 · 207 citations · Full text

, , +16

Targeted mutagenesis in the silkworm Bombyx mori using zinc finger nuclease mRNA injection

Yoko Takasu, Isao Kobayashi, Kelly J. Beumer et al. · Insect Biochemistry and Molecular Biology · 2010 · 161 citations

, , +11

Regio‐ and Stereoselective Steroid Hydroxylation at C7 by Cytochrome P450 Monooxygenase Mutants

Aitao Li, Carlos G. Acevedo‐Rocha, Lorenzo D’Amore et al. · Angewandte Chemie International Edition · 2020 · 136 citations · Full text

, , +17

Rational targeting of a NuRD subcomplex guided by comprehensive in situ mutagenesis

Falak Sher, Mir Ahamed Hossain, Davide Seruggia et al. · Nature Genetics · 2019 · 112 citations

, , +5

1–5 of 40

Authors

249

  1. QW
    Qi Wu

    Zhejiang University, China

    5 publications · 445 citations

    5

    h-index

  2. MT
    Manfred T. Reetz

    Max-Planck-Institut für Kohlenforschung, Germany

    4 publications · 279 citations

    4

    h-index

  3. AL
    Aitao Li

    Hubei University, China

    4 publications · 233 citations

    4

    h-index

  4. JX
    Jian Xu

    Zhejiang University, China

    3 publications · 337 citations

    3

    h-index

  5. JZ
    Jiahai Zhou

    Chinese Academy of Sciences, China

    3 publications · 369 citations

    3

    h-index

1–5 of 249

Institutions

97

  1. Chinese Academy of Sciences

    Beijing, China

    6 h-index

    10 publications

    895 citations

  2. Tianjin Institute of Industrial Biotechnology

    Tianjin, China

    6 h-index

    8 publications

    436 citations

  3. Max-Planck-Institut für Kohlenforschung

    Mülheim, Germany

    6 h-index

    8 publications

    627 citations

  4. Zhejiang University

    Hangzhou, China

    5 h-index

    27 publications

    2.4K citations

  5. Hubei University

    Wuhan, China

    4 h-index

    10 publications

    719 citations

  6. Shanghai Institute of Organic Chemistry

    Shanghai, China

    3 h-index

    7 publications

    945 citations

  7. East China University of Science and Technology

    Shanghai, China

    2 h-index

    11 publications

    231 citations

  8. Philipps University of Marburg

    Marburg, Germany

    2 h-index

    2 publications

    264 citations

1–8 of 97

Venues

  1. Methods in molecular biology

    Book series · 3 publications · 47 citations

    3

    h-index

  2. ACS Catalysis

    3 publications · 142 citations

    3

    h-index

  3. PLoS ONE

    2 publications · 102 citations

    2

    h-index

  4. Biotechnology and Bioengineering

    2 publications · 67 citations

    2

    h-index

  5. Angewandte Chemie International Edition

    2 publications · 169 citations

    2

    h-index

1–5