Concepedia

Dna Shuffling

DNA Shuffling is a molecular biology technique that involves the fragmentation and reassembly of homologous genes to generate chimeric sequences through recombination, thereby enabling the directed evolution of proteins with improved or novel functions.

262

Publications

22.8K

Citations

950

Authors

376

Institutions

Publications per year

2017–2026

43

Parents

Children

Publications

262

Rapid evolution of a protein in vitro by DNA shuffling

Willem P.C. Stemmer · Nature · 1994 · 1.9K citations

, , +10

DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution.

Willem PC Stemmer · Proceedings of the National Academy of Sciences · 1994 · 1.3K citations

, , +15

Improved Green Fluorescent Protein by Molecular Evolution Using DNA Shuffling

Andreas Crameri, Erik A. Whitehorn, Emily Tate et al. · Nature Biotechnology · 1996 · 1.2K citations

, , +11

Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes

Carola Engler, Ramona Gruetzner, Romy Kandzia et al. · PLoS ONE · 2009 · 1.1K citations · Full text

, , +17

DNA shuffling of a family of genes from diverse species accelerates directed evolution

Andreas Crameri, Sun-Ai Raillard, Ericka Bermudez et al. · Nature · 1998 · 828 citations

, , +13

1–5 of 262

Authors

950

  1. WP
    Willem P.C. Stemmer

    Maxygen (United States), United States

    11 publications · 5.3K citations

    11

    h-index

  2. AC
    Andreas Crameri

    Affymax (United States), United States

    5 publications · 2.4K citations

    5

    h-index

  3. FH
    Frances H. Arnold

    California Institute of Technology, United States

    4 publications · 321 citations

    4

    h-index

  4. GL
    Gregory L. Moore

    Xencor (United States), United States

    4 publications · 263 citations

    4

    h-index

  5. AD
    Andrew D. Ellington

    The University of Texas at Austin, United States

    3 publications · 91 citations

    3

    h-index

1–5 of 950

Institutions

376

  1. Maxygen (United States)

    Redwood City, United States

    10 h-index

    29 publications

    8.7K citations

  2. Harvard University

    Cambridge, United States

    8 h-index

    17 publications

    1.3K citations

  3. California Institute of Technology

    Pasadena, United States

    8 h-index

    16 publications

    1.4K citations

  4. Centre National de la Recherche Scientifique

    Paris, France

    8 h-index

    25 publications

    1.1K citations

  5. Pennsylvania State University

    State College, United States

    6 h-index

    15 publications

    1.4K citations

  6. Johns Hopkins University

    Baltimore, United States

    6 h-index

    24 publications

    2.7K citations

  7. Massachusetts Institute of Technology

    Cambridge, United States

    5 h-index

    14 publications

    2K citations

  8. Affymax (United States)

    Cupertino, United States

    5 h-index

    8 publications

    8.2K citations

1–8 of 376

Venues

  1. Nucleic Acids Research

    19 publications · 1.4K citations

    18

    h-index

  2. Proceedings of the National Academy of Sciences

    PNAS · 14 publications · 2.4K citations

    14

    h-index

  3. Journal of Molecular Biology

    JMB · 13 publications · 1.1K citations

    13

    h-index

  4. Gene

    12 publications · 782 citations

    11

    h-index

  5. Nature Biotechnology

    8 publications · 2.2K citations

    8

    h-index

1–5